NCG Mouse

NCG mice are triple immunodeficient and lack functional/mature T, B, and NK cells, and have reduced macrophage and dendritic cell function. These animal models have the ability to host xenograft cells, tissue, and human immune system components.This mouse model is ideal for oncology, immunology, tumor biology, infectious disease, graft-versus-host disease (GvHD), diabetes, regenerative medicine, hematopoiesis and tissue transplant studies. The NCG mouse model can also be utilized to create humanized mice by engrafting PBMCs or CD34 stem cells to closely mimic the human immune system.The NCG mouse is the foundation for further strains and variants including:Pre-humanized CD34+ and PBMC NCG miceThe NCG Plus portfolio of NCG variant mouse models, capable of hosting human tissues and recapitulating the human immune systemOriginCo-developed by Nanjing Biomedical Research Institute of Nanjing University and Nanjing Galaxy Biopharma in 2014 and transferred to Charles River in 2016. The NCG mouse model was created by sequential CRISPR-Cas9 editing of the Prkdc and Il2rg loci in the NOD/Nju mouse, generating a mouse coisogenic to the NOD/Nju.The NOD/Nju carries a mutation in the Sirpa (SIRP α) gene that allows for engrafting of foreign hematopoietic stem cells. The Prkdc knockout generates a SCID-like phenotype lacking proper T-cell and B-cell formation. The knockout of the Il2rg gene further exacerbates the SCID-like phenotype while additionally resulting in a decrease of NK cell production.